STEM CELL BIOLOGY AND REGENERATIVE MEDICINE (WITH LABORATORY)

STEM CELL BIOLOGY AND REGENERATIVE MEDICINE (WITH LABORATORY)

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iten
Code
98798
ACADEMIC YEAR
2019/2020
CREDITS
6 credits during the 1st year of 10598 MEDICAL-PHARMACEUTICAL BIOTECHNOLOGY (LM-9) GENOVA
SCIENTIFIC DISCIPLINARY SECTOR
BIO/13
LANGUAGE
English
TEACHING LOCATION
GENOVA (MEDICAL-PHARMACEUTICAL BIOTECHNOLOGY)
semester
1° Semester
Prerequisites
Teaching materials

OVERVIEW

This class will offer comprehensive lectures on the basics in stem cell biology as well as more specific information on  functional characterisation of different stem cell populations (from embryonic to adult stem cells, including fetal, perinatal and tissue-specific progenitors, up to induced pluripotent and cancer stem cells) and their relevance in regenerative medicine. Ageing and injury response mechanisms will be discussed along with stem cell trans-differentiation over paracrine potential.

AIMS AND CONTENT

LEARNING OUTCOMES

Analysis of stem cell biology, focusing on their differentiation and therapeutic potential (including somatic nuclear transfer and cloning).

AIMS AND LEARNING OUTCOMES

This class will help students to develop knowledge and specific understanding and a strong foundation in stem cell biology and their therapeutic potentail. Lessons will be delivered from the Lecturer in English to help students develop their communicative skills.

By attending class and by studying on their own, students will be able to develop:

- detailed comprehension on specific functional and regenerative properties of different stem cell populations;

- specific knowledge on mechanisms controlling stem/progenitor cell activation (i.e. EMT transition), differentiation potential (i.e. therapeutic cloning and reprogramming) and paracrine secretion for intercellular communication;

- develop comprehensive know-how, while improving their learning and communication skills along with capacity of critical analysis on aspects mostly relevant for future translational medicine.

This course will also support students to further acquire and develop their communicative skills as presenting in English and discussing in front of class some original reserach articles during a journbal club activity to be perfomed as team of 2-3 students at the end of the course.

The laboratory activity will foster students' ability to engage into pratical cell biology research by practising techniques and protocols relevant to the main topics discussed in class (i.e. primary isolation of human stem cells versus differentiated somatic ones, isolation and charachterization of cell-secreted extracellular vesicles, immunofluorescence analyses, etc).

PREREQUISITES

Students are required to posses basic to proficient comptence in English, both as comprehensive and communicative skill. Validated knowledge on basic cell biology and on mechanisms of gene expression and regulation is also necessary.

Teaching methods

Students are required to attend class for at least 75% of the scheduled lessons.

The module will include 36 hours of lecturing; each lecture will take place twice a week. A practical course of hands on in laboratory techniques will also be performed for a total of 32 hours within 8 days, in the afternoon (2-6pm), after lecturing will be concluded. During the laboratory activity, students will be supervised by the Lecturer with the assistance of young reserachers (with a PhD and/or Master of Science degree) with broad expertise on the specific topics.

Class will be managed as frontal lectures with Power Point notes. The main topics will be addressed during class by means of active discussion engaged by the Lecturer.

Students can take advantage of online material (lectures power point notes) available on the AulaWeb website; such material must not be shared to a third party, neither posted online or made available on any social/sharing platform.

 Lecturer can be reached via email/phone to arrange specific 1-to-1 meeting for further assistance/explanation as needed.

SYLLABUS/CONTENT

Introduction on stem cell biology.

Embryonic stem cells: functional characterisation and their applications in preclinical research.

Adul stem cells: hematopoietic, neural and epithelial stem cells. Mesenchymal stromal stem cells: tissue origin and their regenerative potential.

Somatic nuclear transfer from amphibians to mammals. Therapeutic cloning.

Induced pluripotent stem cells and reprogramming. Pros and cons in preclinical research and their translational potential.

Fetal and perinatal stem cells: definition of their regenerative potential compared to embryonic and adult stem cells.

Phenotypic and functional characterisation of cancer stem cells.

EMT: Epithelial - Mesenchymal Transition in cell biology.

Tissue-specific adult progenitor cells within the cardiac tissue; an example of EMT in stem cell biology: the epicardium-derived progenitor cells (EPDC).

Tissue-specific adult progenitor cellswithin the skeletal muscle. 

Stem cells, DNA damage & ageing.

Intercellular communication part I: stem cells and paracrine effect in regenerative medicine

Intercellular communication part II: stem cell-secreted extracellular vesicles in regenerative medicine.

Regenerative medicine 2.0: de-differentiation, trans-differentiation and direct reprogramming.

Journal Club: Oral presentation from groups of students critically discussing assigned research articles related to the topics discussed during class.

Hands On Practice on Cell Biology Laboratory

Multipotent stem cell in vitro culture versus tissue-specific differentiated cell isolation. Isolation and assessment of protein concentration of stem cell secretome. Isolation and general characterisation. Of stem cell-derived extracellular vesicles. microRNA isolation from  circulating extracellular vesicle within blood samples.

 

RECOMMENDED READING/BIBLIOGRAPHY

Lecturers will provided power point slides at the end of the class as available to be downloaded from the AulaWeb website, as supporting material, which must not be either disclosed to third or shared on line or posted on social media. Laboratory protocols will be also made available before the practical hands on course starts.

For the journal club activity, Lecturers will made available for students to choose some original research articles published on peer-reviewed international journals; students will work on them in groups of 2-3 people to deliver an oral presentation in order to critical discuss the chosen study in front of class.

 

 

TEACHERS AND EXAM BOARD

Ricevimento: Soon after lecture hours or by appointment, please contact the Lecturer by email: sveva.bollini@unige.it or roberta.tasso@unige.it The office address is: Biology Section, Dept. Experimental Medicine, Via Pastore 3, second floor. E-mail appointments are strongly encouraged. Alternatively the teacher can be reached at the following phone number: 01035350080/0105558257/0105558394.

Ricevimento: Soon after lecture hours or by appointment, please contact the Lecturer by email: roberta.tasso@unige.it. The office address is: Biology Section, Dept. Experimental Medicine, Via Pastore 3, second floor. E-mail appointments are strongly encouraged. Alternatively the teacher can be reached at the following phone number: 0105558319 - 0105558319.

Exam Board

ROBERTA TASSO (President)

SVEVA BOLLINI (President)

LESSONS

Teaching methods

Students are required to attend class for at least 75% of the scheduled lessons.

The module will include 36 hours of lecturing; each lecture will take place twice a week. A practical course of hands on in laboratory techniques will also be performed for a total of 32 hours within 8 days, in the afternoon (2-6pm), after lecturing will be concluded. During the laboratory activity, students will be supervised by the Lecturer with the assistance of young reserachers (with a PhD and/or Master of Science degree) with broad expertise on the specific topics.

Class will be managed as frontal lectures with Power Point notes. The main topics will be addressed during class by means of active discussion engaged by the Lecturer.

Students can take advantage of online material (lectures power point notes) available on the AulaWeb website; such material must not be shared to a third party, neither posted online or made available on any social/sharing platform.

 Lecturer can be reached via email/phone to arrange specific 1-to-1 meeting for further assistance/explanation as needed.

LESSONS START

October 1st 2019, according to the scheduled made available from the organizing secretary

EXAMS

Exam description

Details about final exam will be explained during class. Assessment will be performed via a 1-to-1 discussion in English with the Lecturers about the main topics illustrated during class.

There will be 7 exams per year: 3 during the winter session (January and February) and 4 during the summer session (3 in June-July and one in September). Extraordinary dates will NOT be arranged.

Online registration with at least 48h advance is mandatory to have access to the exam.

In the final mark computation, Lecturers will also take into consideration the journal club activity.

 

Assessment methods

During the oral interview the student's comprehension on the specific topics discussed in class. Students will be assessed considering their knowledge and understanding. Their communicative and making judgments skills will be evaluated as well.

During the course, students will be also asked to critically discuss during the Journal Club activity some research articles related to the main topic illustrated during class by an oral presentation, as the result of their team work attitude with their colleagues. 

Exam schedule

Date Time Location Type Notes
14/09/2020 14:00 GENOVA Orale c/o Aula Torre A piano 3 CBA.

FURTHER INFORMATION

For any further clarifications and/or explanation or any required information, students are strongly invited to contact the Lecturers - Prof. Bollini and Prof. Tasso-  in advance by email (sveva.bollini@unige.it and roberta.tasso@unige.it) or phone, to arrange specific appointments.