BIOTECHNOLOGY DIAGNOSTIC AND NANOTECHNOLOGY AND LABORATORY

BIOTECHNOLOGY DIAGNOSTIC AND NANOTECHNOLOGY AND LABORATORY

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iten
Code
95303
ACADEMIC YEAR
2018/2019
CREDITS
7 credits during the 2nd year of 10598 MEDICAL-PHARMACEUTICAL BIOTECHNOLOGY (LM-9) GENOVA
SCIENTIFIC DISCIPLINARY SECTOR
BIO/10
LANGUAGE
English
TEACHING LOCATION
GENOVA (MEDICAL-PHARMACEUTICAL BIOTECHNOLOGY)
semester
1° Semester
Teaching materials

OVERVIEW

The course of Diagnostic Biotechnology and Nanotechnology will offer an overview of the techniques and biotechnologies applied to diagnostics, from the most classic and consolidated to those most innovative and recently developed, also with nanotechnological approaches.

AIMS AND CONTENT

LEARNING OUTCOMES

Module “Diagnostic Biotechnologies” To introduce students to the methods derived from basic biotechnologies that were transferred to medical diagnostics To know the main analyses of clinical biochemistry and their interpretation to verify metabolism and function of different organs Module “Nanotechnology” The aim of the course is to give to the students an overview of the most recent and advanced nanobiotechnologies and nanobiomaterials. In particular, the two aspects of nanobioscience and nanobiotechnology application take place: structural proteomics and functional proteomics. In the first case, the novel method of protein nanocrystallography and the advanced synchrotron radiation techniques, including micro and nano-focussed high intense synchrotron radiation and the new trends in radiation damage reduction are presented. In the second case, the most innovative technologies of protein microarrays NAPPA (Nucleic Acid Programmable protein Array) and their fluorescent-label as well as label-free technology of are demonstrated. In the both cases the application to the regenerative medicine are underlined.

AIMS AND LEARNING OUTCOMES

-To learn the phases of the diagnostic pathway (pre-analytical phase, analytical phase and post analytical phase) and the degree of reliability of the obtained analytical results.

-To know the main analyses of clinical biochemistry exploited in medical diagnostics, to interpret the results to verify metabolism and function of different organs. To evaluate which tests are more appropriate to determine a specific diagnosis. To explain the methodological principles of the main laboratory tests.

-To know the most recent diagnostic applications of genomic biotechnologies, of cytofluorimetry, of nanoparticles for innovative nanosensors.

-To learn principles of micro/nanoscopy (super-resolution) and their potential applications.

Teaching methods

Frontal teaching. Seminars held by experts on specific subjects (Prof Fenoglio, expert in cytofluorimetry).

Laboratories

-Experience in the Analysis Laboratories (San Martino Hospital)

-Experience in a lab at IIT for preparation of nanoparticles for diagnostics use

Presentation of specific in-depth studies prepared by the students, individually or in groups (optional).

SYLLABUS/CONTENT

- Management systems of biotech industry and their use in medical diagnostics laboratories.

- The pre-analytical, analytical and post analytical phase, in diagnostics. The analytical method: accuracy, precision, specificity, sensitivity; internal quality control and external quality assessment.  controllo di qualità interno ed esterno. Reference values and range in reports.

- Main laboratory test in clinical biochemistry (including the used technology):

  1. Blood: hemochromocytometric test; electrophoretic profile of serum proteins; plasma enzymes in diagnosis; non-protein nitrogen.
  2. Hepatic function tests: enzymes (AST, ALT, GGT, AP), bilirubin, serum proteins.
  3. Glucose metabolism test: hormones regulating glycemia; causes for hyper- and hypo-glycemia; diagnostic parameter for diabetes mellitus; laboratory tests in diabetic ketoacidosis; glucose tolerance test; glycosylated hemoglobin; ketonic compounds.
  4. Lipid metabolism test: lipoprotein and apolipoprotein classification and their metabolism; reference value for different classes of lipids; dyslipidemic phenotypes.
  5. Urine analysis and renal function test: physical chemical urine analysis; renal function test (creatinine clearance, BUN-blood urea nitrogen, urine concentration capacity.
  6. Endocrinology: hints to the function of the main hormones, basal levels and conditions of hyper-/hypo-secretion; laboratory tests in the different hyper-/hypo-secretion conditions.

- innovative diagnostic applications of cytofluorimetry.

- Microscopy/nanoscopy for viasualization and analysis of biological systems at cellular and molecular levels in 2D and 3D: potential diagnostic applications in molecular oncology and neurodegenerative diseases.

-Development of innovative nanosensors, based on nanoparticles, for the detection of biomolecules and analytes of diagnostic interest.

RECOMMENDED READING/BIBLIOGRAPHY

Notes/slides prepared and provided by the teachers.

TEACHERS AND EXAM BOARD

Ricevimento: By appointment by email: santina.bruzzone@unige.it Office: DIMES, Section  of Biochemistry, Viale Benedetto XV, 1 (Genova) Phone: 0103538161  

Exam Board

SANTINA BRUZZONE (President)

MICHELA TONETTI

LESSONS

Teaching methods

Frontal teaching. Seminars held by experts on specific subjects (Prof Fenoglio, expert in cytofluorimetry).

Laboratories

-Experience in the Analysis Laboratories (San Martino Hospital)

-Experience in a lab at IIT for preparation of nanoparticles for diagnostics use

Presentation of specific in-depth studies prepared by the students, individually or in groups (optional).

LESSONS START

First semester, according to the timetable.

EXAMS

Exam description

Written exam, as a test with multiple choice.

The exam on Prof Visconti’s part can be taken separately, or concomitantly to Prof Bruzzone/Prof Pompa’s part. Successful result from passing one of the two parts will be valid, without limit.   The final mark will be obtained by the average, weighted according to the credits of the two parts. In addition, the ability of presenting the in-depth study (optional), as well as the shown interest and participation to the laboratory experiences, will contribute to the definition of the final mark.

Assessment methods

The written exam will evaluate the achievement of the aims, asking questions, for example: on methodologies (verification of the knowledge of the principles of some techniques); on the identification of the most suitable test for the answer to a medical question; on the comparison between values obtained in different analyzes; on the interpretation of clinical results.