FLOW CYTOMETRY: BASIC PRINCIPLES AND BIOLOGICAL APPLICATIONS

iten
Code
96332
ACADEMIC YEAR
2017/2018
CREDITS
2 credits during the 2nd year of 9015 Molecular and health biology (LM-6) GENOVA
SCIENTIFIC DISCIPLINARY SECTOR
MED/09
LANGUAGE
Italian
TEACHING LOCATION
GENOVA (Molecular and health biology)
semester
1° Semester

OVERVIEW

Flow cytometry is a widely used analytical method in research laboratories and not only.  It allows to analyze a large number of cells in a short time by quantifying multiple parameters for each cell. In the field of biological research, flow cytometry has become fundamental, enabling a major advance in understanding cellular dynamics and cellular communication. 

AIMS AND CONTENT

LEARNING OUTCOMES

The course aims at providing basic training with a broad dissemination technique
In particular, the course aims at achieving the following objectives:
- knowledge of the principles on which flow cytometry and instrumentation are based;
- learning of basic techniques;
- deepening of some applications of common use;
- reading and interpreting the obtained results

TEACHING METHODS

Lessons and discussion of cytometric files

SYLLABUS/CONTENT

Basic principles of fluorescence and light-particle interaction

Basic principles of cytometry: General concepts underlying the operation of the flow cytometer and what are the main optical parameters measurable in cytometry

Instrumentation: - Optical part, fluidic part, electronic part

Instrumentation - part of setting: Instrumentation control procedures (calibration, calibration, linearity); Setting the threshold of analysis, compensation

Sample preparation: basic knowledge on the most important techniques for preparing the samples to be analyzed; Fresh, fixed or frozen samples

Fluorescent Cytometry Probes: General information on colorants for nucleic acids, proteins, and the measurement of key cellular features.

Management and Analysis of Generated Data: Principal concepts related to the analysis of a cytometric file: notions about the different gating modes; Off-line compensation of fluorescents; Isotypic and isoclonic control, fluorescence minus one (FMO); Criteria for the assessment of positivity; Main typologies of cytometric data representation.

Cytometric Platform Applications: surface markers, intracytoplasmic and intranuclear markers; Evaluation of cell proliferation; Apoptosis assessment; Cell signaling; cytotoxicity; Polychromatic cytometry; Manipulation of cultured cell lines and their preparation for cytometric assays (with particular reference to adhesion cultures: detachment, fixation, etc.); Treatment and analysis of solid tissue samples (biopsies) with particular reference to the various disaggregation techniques; Microvescicole study

TEACHERS AND EXAM BOARD

Office hours: The interview with the teacher can be obtained by email (daniela.fenoglio@unige.it) or by phone (010 / 3533027-3029). The teacher's studio is located in the Directorate of the Centre of Excellence for Biomedical Research (CEBR) - Viale Benedetto XV, 7 -16132 Genova ​

Exam Board

DANIELA FENOGLIO (President)

ALESSIA PARODI

LESSONS

TEACHING METHODS

Lessons and discussion of cytometric files

Class schedule

All class schedules are posted on the EasyAcademy portal.

EXAMS

Exam schedule

Date Time Location Type Notes
31/01/2018 09:00 GENOVA Orale
16/02/2018 09:00 GENOVA Orale
11/06/2018 09:00 GENOVA Orale
02/07/2018 09:00 GENOVA Orale
19/07/2018 09:00 GENOVA Orale
03/09/2018 09:00 GENOVA Orale
26/09/2018 09:00 GENOVA Orale