CELL CULTURES AND LABORATORY

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Code
65539
ACADEMIC YEAR
2017/2018
CREDITS
2 credits during the 3nd year of 8762 Biological Sciences (L-13) GENOVA
SCIENTIFIC DISCIPLINARY SECTOR
BIO/06
LANGUAGE
Italian
TEACHING LOCATION
GENOVA (Biological Sciences)
semester
2° Semester
Prerequisites
Prerequisites
You can take the exam for this unit if you passed the following exam(s):
  • Biological Sciences 8762 (coorte 2015/2016)
  • CYTOLOGY AND HISTOLOGY AND LABORATORY 65513
  • ANIMAL PHYSIOLOGY AND LABORATORY 67060

AIMS AND CONTENT

LEARNING OUTCOMES

Provide students with an in-depth understanding of the techniques used to grow and manipulate "in vitro" cells and the use of different types of cell culture.

TEACHING METHODS

lesson

SYLLABUS/CONTENT

Cells in vitro. Primary culture, cell lines. Basic techniques of cell culture in vitro. Advantages and disadvantages of cell cultures. The 3 Rs. Needed equipment and sterilization methods. Composition of different media. Tripsinisation and propagation of crops. Freezing and thawing. Cellular adhesion. Growth on specific substrates (polyglycine, polyornitine, collagen). Growth kinetics. Cellular count. Methods for evaluating cell proliferation, survival and toxicity. Vitality Test. Some applications: wound healing 

 

The Laboratory credit will be devoted to the practical application of what explained during the theoretical lessons (setting up a culture, cell count, subculturing, wound healing)

RECOMMENDED READING/BIBLIOGRAPHY

  • G.L. Mariottini et al. Introduzione alle colture cellulari: metodiche relative – MORGAN ed. tecniche
  • Biochimica e biologia molecolare. Principi e tecniche
  • Keith Wilson,John Walker Ed. RaffaelloCortina
  • - Material provided by the teacher

TEACHERS AND EXAM BOARD

Office hours: On appointment

Exam Board

ELENA GRASSELLI (President)

ILARIA DEMORI

ADRIANA VOCI

LESSONS

TEACHING METHODS

lesson

LESSONS START

To agree with the students

Class schedule

All class schedules are posted on the EasyAcademy portal.

EXAMS

EXAM DESCRIPTION

oral

Exam schedule

Date Time Location Type Notes
22/01/2018 09:30 GENOVA Orale
20/06/2018 09:30 GENOVA Orale
19/07/2018 09:30 GENOVA Orale
27/09/2018 09:30 GENOVA Orale